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. 1975 Feb;72(2):418-22.
doi: 10.1073/pnas.72.2.418.

A new complement function: solubilization of antigen-antibody aggregates

G W Miller, V Nussenzweig

A new complement function: solubilization of antigen-antibody aggregates

G W Miller et al. Proc Natl Acad Sci U S A. 1975 Feb.

Abstract

Antigen-antibody aggregates are solubilized when incubated with fresh serum at 37 degrees, yielding immune-complexes of relatively small molecular weight which contain antigen, antibody, and complement (C3)determinants. Solubilization is complement-dependent,requires free Mg++ but not Ca++, and proceeds in sera from C4- or C5-deficient animals. It is accelerated in the presence of Ca++ in normal or C4-deficient guinea pig serum, suggesting involvement of the Cl-bypass activation of the properdin system. Immune precipitates can also be solubilized by monovalent fragments (Fab) of antibodies directed against determinants of the antibody molecules included in the antigen-antibody lattice. Similarly, it is suggested that complement-mediated solubilization might be induced by the combination of a complement fragment with the antibody in the immune-aggregate.

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