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. 1975 Feb;72(2):581-5.
doi: 10.1073/pnas.72.2.581.

Isolation of the bacteriophage lambda A-gene protein

A Becker, M Gold

Isolation of the bacteriophage lambda A-gene protein

A Becker et al. Proc Natl Acad Sci U S A. 1975 Feb.

Abstract

An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.

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