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Comparative Study
. 1975 Feb;72(2):619-22.
doi: 10.1073/pnas.72.2.619.

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin

J R Benson, P E Hare
Comparative Study

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin

J R Benson et al. Proc Natl Acad Sci U S A. 1975 Feb.

Abstract

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.

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References

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