Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Feb;72(2):701-5.
doi: 10.1073/pnas.72.2.701.

Translation in vitro of rat brain messenger RNA coding for tubulin and actin

I Gozes, H Schmitt, U Z Littauer

Translation in vitro of rat brain messenger RNA coding for tubulin and actin

I Gozes et al. Proc Natl Acad Sci U S A. 1975 Feb.

Abstract

A partially purified fraction of poly(a)-rich brain mRNA coding for tubulin and actin was obtained by stepwise elution from an oligo(deoxythymidylate)-cellulose column and was efficiently translated in a wheat-germ cell-free system. The newly synthesized tubulin and actin migrated along with the authentic proteins on sodium dodecyl sulfate polyacrylamide gels and on sodium dodecyl sulfate-urea polyacrylamide gels, where tubulin alpha- and beta-subunits are separated. The two proteins synthesized in vitro were found to be biologically active; they could be induced to polymerize and were both precipitated by vinblastine. In addition, specific binding of tubulin to an affinity column of colchicine-Sepharose and actin to myosin were demonstrated.

PubMed Disclaimer

References

    1. FEBS Lett. 1972 Jul 15;24(1):113-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4467-71 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 - PubMed
    1. Eur J Biochem. 1974 Jul 1;46(1):83-8 - PubMed

LinkOut - more resources