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. 1975 Feb;72(2):742-5.
doi: 10.1073/pnas.72.2.742.

5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA

Y Furuichi, S Muthukrishnan, A J Shatkin

5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA

Y Furuichi et al. Proc Natl Acad Sci U S A. 1975 Feb.

Abstract

Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.

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References

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