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Comparative Study
. 1999 Nov;6(6):924-9.
doi: 10.1128/CDLI.6.6.924-929.1999.

Use of monoclonal antibody in diagnosis of candidiasis caused by Candida albicans: detection of circulating aspartyl proteinase antigen

B K Na  1 , C Y Song
Affiliations
Comparative Study

Use of monoclonal antibody in diagnosis of candidiasis caused by Candida albicans: detection of circulating aspartyl proteinase antigen

B K Na et al. Clin Diagn Lab Immunol. 1999 Nov.

Abstract

To develop a serological diagnosis of invasive candidiasis based on detection of circulating secreted aspartyl proteinase (SAP) antigen of Candida albicans, three different enzyme-linked immunosorbent assays (ELISAs) were compared. The first was a standard ELISA to detect anti-SAP antibodies, and the others were an antigen capture ELISA and an inhibition ELISA to detect circulating SAP antigen with monoclonal antibody (MAb) CAP1, which is highly specific for SAP. These tests were applied to 33 serum samples retrospectively selected from 33 patients with mycologically and/or serologically proven invasive candidiasis caused by C. albicans. Serum samples from 12 patients with aspergillosis and serum samples from 13 healthy individuals were also included. The sensitivities and specificities were 69.7 and 76.0% for the standard ELISA and 93.9 and 92.0% for the antigen capture ELISA, respectively. However, these values reached 93.9 and 96.0%, respectively, for the inhibition ELISA. Serum samples from 31 of 33 patients had detectable SAP antigen, with concentrations ranging from 6.3 to 19.0 ng/ml. These results indicate that the inhibition ELISA with MAb CAP1 is effective in detection of circulating SAP antigen and that this assay may be useful for diagnosis and treatment monitoring of invasive candidiasis.

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Figures

FIG. 1
FIG. 1
Amount of SAP detected by antigen capture ELISA. Data are averages calculated from triplate OD obtained with three different batches of in vitro-produced SAP diluted in PBS. Standard deviations were artificially calculated from calibration curves with OD corresponding to different SAP concentrations.
FIG. 2
FIG. 2
Amount of SAP detected by inhibition ELISA. Data are averages calculated from triplate OD obtained with three different batches of in vitro-produced SAP diluted in PBS. Standard deviations were artificially calculated from calibration curves with OD corresponding to different SAP concentrations.
See this image and copyright information in PMC

References

    1. Bodey G P, Fainstein V. Candidiasis. In: Bodey G P, Fainstein V, editors. Candidiasis. New York, N.Y: Raven Press; 1985. pp. 53–54.
    1. Bodey G P, Anaissie E J. Chronic systemic candidiasis. Eur J Clin Microbiol Infect Dis. 1989;8:855–857. - PubMed
    1. Borg M, Rüchel R. Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infect Immun. 1988;56:626–631. - PMC - PubMed
    1. Buckley H R, Richardson M D, Evans E G V, Wheat L J. Immunodiagnosis of invasive fungal infection. J Med Vet Mycol. 1992;30(Suppl. 1):249–260. - PubMed
    1. Chakrabarti A, Roy P, Kumar D, Sharma B K, Chugh K S, Panigrahi D. Evaluation of three serological tests for detection of anti-candidal antibodies in diagnosis of invasive candidiasis. Mycopathologia. 1994;126:3–7. - PubMed

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