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. 1999 Oct 15;858(2):239-44.
doi: 10.1016/s0021-9673(99)00841-9.

Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana

S Bala  1 , G C Uniyal, S K Chattopadhyay, V Tripathi, K V Sashidhara, M Kulshrestha, R P Sharma, S P Jain, A K Kukreja, S Kumar
Affiliations

Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana

S Bala et al. J Chromatogr A. .

Abstract

A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2'-deacetoxydecinnamoyltaxinine J and 2'-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV-Vis as well as photodiode array detection.

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