doi: 10.1073/pnas.72.3.777.
Crystallization of nerve growth factor from mouse submaxillary glands
- PMID: 1055377
- PMCID: PMC432402
- DOI: 10.1073/pnas.72.3.777
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Crystallization of nerve growth factor from mouse submaxillary glands
Proc Natl Acad Sci U S A.
1975 Mar.
Abstract
Crystals of the nerve growth factor protein were grown by vapor diffusion from ethanol solution. The crystals are hexagonal, belonging to space group P622 (or its enantiomorph) with a equals 56.1 A, c equals 181.4 A, and V equals 494,400 A. The unit cell contains six molecules of dimeric protein and thus has one monomer per asymmetric unit. The diffraction pattern extends to at least 2.7 A, indicating that this crystal form is suitable for structural analysis to near-atomic resolution.
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