Purification and characterization of the retina-specific cell-aggregating factor
- PMID: 1055390
- PMCID: PMC432432
- DOI: 10.1073/pnas.72.3.916
Purification and characterization of the retina-specific cell-aggregating factor
Abstract
The tissue-specific, cell-aggregating component of embryonic neural retina cells was purified from the retina cell-aggregating factor and characterized as a glycoprotein. Its molecular weight in solution is in the range of 50,000, and it contains 10-15% carbohydrate. The amino-acid and carbohydrate compositions have been determined. The glycoprotein is produced by embryonic neural retina cells in primary monolayered cultures and is released into the medium. Its tissue-specific, cell-aggregating effect requires integrity of the polypeptide portion, but not of the carbohydrate portion. We suggest that the isolated molecule is a specific determinant of the embryonic retina cell-surface and that it is involved in mediating self-recognition and selective adhesiveness of these cells.
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