[Effects of 5-hydroxytryptamine creatinine sulfate on development of the in vitro cultivated explants of the cerebrocortex of neonatal rats (author's transl)]

Abstract

Explants of the cerebrocortex of neonatal rats were cultivated up to 15 DIV in addition to 5-hydroxytryptamine creatinine sulfate at the final concentration of 0.1 mM. Fluorescence histochemical investigations were accomplished with a modified glyoxylic acid "heat reaction" according to Lindvall and Björklund (1974). Depending on the duration of the cultivation and/or influence of the 5-hydroxytryptamine creatinine sulfate in varied fiber and cell elements of the explants varied effects were found. The retardation of the growth was striking. Yellow-greenish fluorescence is interpreted as a fluorescence of the metabolic substances of the serotonin. Cells with a brownish-yellow fluorescence typical of 5-hydroxytryptamine after treatment with glyoxylic acid in the centre of the explants were found. Analogous to the findings of the neuroembryology in different species in situ the results were interpreted with respect to role of the neurotransmitters as modulators of the neurogenesis.