Reversed-phase high-performance liquid chromatographic measurement of the P-aminobenzoic acid synthesized by Streptococcus sanguis

Abstract

Objective: This study investigated the biosynthesis of P-aminobenzoic acid by Streptococcus sanguis and the role of P-aminobenzoic acid in the interaction between Streptococcus sanguis and Streptococcus mutans.

Methods: A reversed-phase high-performance liquid chromatographic technique was used to analyze the P-aminobenzoic acid synthesized by Streptococcus sanguis in an anaerobic atmosphere.

Results: The results showed, that (1) the chromatographic method established in this study had good linear relation and the average recovery of P-aminobenzoic acid was 80%, and (2) Streptococcus sanguis did synthesize P-aminobenzoic acid, and the mean concentration of P-aminobenzoic acid was 1.23 micrograms/mL.

Conclusions: The results of this study will help further studies of the factors that may have effects on P-aminobenzoic acid synthesis by Streptococcus sanguis and on the role of P-aminobenzoic acid in the microbial homeostasis of dental plaque.