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. 1999 Jan;124(1):61-6.
doi: 10.1039/a807226d.

Simultaneous determination of cis- and trans-resveratrol in wines by capillary zone electrophoresis

J J Berzas Nevado  1 , A M Contento Salcedo, G Castañeda Peñalvo
Affiliations

Simultaneous determination of cis- and trans-resveratrol in wines by capillary zone electrophoresis

J J Berzas Nevado et al. Analyst. 1999 Jan.

Abstract

A capillary zone electrophoresis method for determining cis- and trans-resveratrol isomers is proposed. Optimal conditions for the quantitative separation were investigated. A background electrolyte solution consisting of 40 mM borate buffer adjusted to pH 9.5, hydrodynamic injection and 5 kV of separation voltage were used. Good linearity and precision were obtained for the two isomers. Detection limits of 0.06 mg L-1 for trans-resveratrol and 0.08 mg L-1 for cis-reveratrol were obtained. The developed method is rapid and sensitive and it has been applied to determine cis- and trans-resveratrol in several red wines. The samples were purified and enriched by passing them through a preconditioned C18 cartridge and eluting the isomers with acetonitrile-water (3 + 7).

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