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. 1999 Dec;37(12):4131-4.
doi: 10.1128/JCM.37.12.4131-4134.1999.

Characteristics of Streptococcus pyogenes serotype M1 and M3 isolates from patients in Japan from 1981 to 1997

T Murase  1 , R Suzuki, R Osawa, S Yamai
Affiliations

Characteristics of Streptococcus pyogenes serotype M1 and M3 isolates from patients in Japan from 1981 to 1997

T Murase et al. J Clin Microbiol. 1999 Dec.

Abstract

Streptococcus pyogenes isolates obtained in 1981 to 1997 from patients and healthy subjects were characterized by pulsed-field gel electrophoresis (PFGE) patterns, biotyping, and the presence of spe genes encoding streptococcal pyrogenic exotoxins. Changes in the profiles were shown in the serotype M1/T1 isolates from pharyngitis over this period, but not in serotype M3/T3 isolates. The characteristics of isolates from patients with toxic shock-like syndrome (TSLS) were comparable to those of the other isolates, including those from healthy subjects. This finding suggests that further phenotypic and molecular characterization, such as investigating the genomic difference represented by the pathogenicity island, of isolates with apparently the same profiles would be necessary to determine the etiology of diseases caused by S. pyogenes, including TSLS.

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Figures

FIG. 1
FIG. 1
(A) Representative PFGE patterns of SmaI-digested chromosomal DNA of S. pyogenes isolates. Lanes: 1, type Ia; 2, type IIa; 3, type IIIa; M, lambda ladders. Arrows indicate the fragments which are common within each of the PFGE types (see text). (B) SfiI-digested patterns of S. pyogenes isolates. Lanes: 1 and 18, lambda DNA ladder; 2 and 19, Saccharomyces cerevisiae DNA; 3, type A1; 4, type A2; 5, type A3; 6, type A4; 7, type A5; 8, type B1; 9, type B2; 10, type B3; 11, type B4; 12, type E; 13, type C1; 14, type C2; 15, type C3; 16, type D; 17, type F. The sizes of the markers (in kilobase pairs) are indicated to the left of each panel.
FIG. 2
FIG. 2
Schematic diagram showing the differences between the PFGE patterns and the predominant patterns in each of the serotypes—types Ia (A), IIa (B), and IIIa (C). Triangles indicate fragments present in the predominant patterns and missing from those patterns after a possible genetic event(s); asterisks indicate the fragment is absent from the predominant patterns, but present in the patterns after a possible genetic event(s).
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