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. 1975 May;72(5):1758-62.
doi: 10.1073/pnas.72.5.1758.

Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum

M Clarke, G Schatten, D Mazia, J A Spudich

Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum

M Clarke et al. Proc Natl Acad Sci U S A. 1975 May.

Abstract

Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.

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