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. 1999 Nov;37(11):831-840.
doi: 10.1016/s0981-9428(99)00115-1.

The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase

JM Routaboul  1 , C Benning, N Bechtold, M Caboche, L Lepiniec
Affiliations

The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase

JM Routaboul et al. Plant Physiol Biochem. 1999 Nov.

Abstract

Diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) is a membrane enzyme that drives the final step in the formation of oils using diacylglycerol (DAG) and acyl-CoA to yield triacylglycerol (TAG). We identified a putative plant DGAT gene (TRIACYLGLYCEROL1: TAG1) and demonstrated its function by the cloning of two mutated alleles, designated AS11 (tag1-1) and ABX45 (tag1-2). One allele, AS11, has been previously characterised at the biochemical level. Mutant seeds contained less oil with a modified fatty acid profile and have reduced germination rates compared to wild-type controls. The TAG1 cDNA encodes for a 520-aa protein that possesses multiple putative transmembrane domains and shows 70 % similarity to a human DGAT cDNA.

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