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Comparative Study
. 1999 Dec 15;65(2):187-93.
doi: 10.1016/s0168-1702(99)00117-3.

Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3' untranslated region identifies the virus as a close relative of infectious bronchitis virus

J J Breslin  1 , L G Smith, F J Fuller, J S Guy
Affiliations
Comparative Study

Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3' untranslated region identifies the virus as a close relative of infectious bronchitis virus

J J Breslin et al. Virus Res. .

Abstract

The 3' end of the turkey coronavirus (TCV) genome (1740 bases) including the nucleocapsid (N) gene and 3' untranslated region (UTR) were sequenced and compared with published sequences of other avian and mammalian coronaviruses. The deduced sequence of the TCV N protein was determined to be 409 amino acids with a molecular mass of approximately 45 kDa. The TCV N protein was identical in size and had greater than 90% amino acid identity with published N protein sequences of infectious bronchitis virus (IBV); less than 21% identity was observed with N proteins of bovine coronavirus and transmissible gastroenteritis virus. The 3' UTR showed some variation among the three TCV strains examined, with two TCV strains, Minnesota and Indiana, containing 153 base segments which are not present in the NC95 strain. Nucleotide sequence identity between the 3' UTRs of TCV and IBV was greater than 78%. Similarities in both size and sequence of TCV and IBV N proteins and 3' UTRs provide additional evidence that these avian coronaviruses are closely related.

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Figures

Fig. 1
Fig. 1
Comparison of amino acid sequences of the nucleocapsid protein of TCV (Minnesota, Indiana and NC95 strains) and published sequences of IBV (Beaudette, KB8523 and M41 strains; Boursnell et al., 1985, Sutou et al., 1988). The positions where amino acids are identical are indicated as (·).
Fig. 2
Fig. 2
Comparison of nucleotide sequences of the 3′ untranslated region of TCV (Minnesota, Indiana, and NC95 strains) and published sequences of IBV (Beaudette and M41 strains; Boursnell et al., 1985, Sutou et al., 1988). The positions where nucleotides are missing are indicated as (–) and identical nucleotides are indicated as (·).
See this image and copyright information in PMC

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