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. 1978 Oct 30;287(11):1043-6.

[Purfication and molecular weight of an RNA-dependant RNA polymerase from Brassicae oleracea var. Botrytis]

[Article in French]
S Astier-Manifacier, P Cornuet
  • PMID: 105817

[Purfication and molecular weight of an RNA-dependant RNA polymerase from Brassicae oleracea var. Botrytis]

[Article in French]
S Astier-Manifacier et al. C R Acad Hebd Seances Acad Sci D. .

Abstract

An RNA-dependent RNA polymerase has been completely purified from Cauliflower inflorescences. Analysis of the purified enzyme on SDS-polyacrylamide gels showed one polypeptide chain with a molecular weight of 140,000 dalton. The enzyme is monomeric in its native state. The in vitro activity was completely dependent on added RNA, the most efficient templates being poly (U), poly (U, C), poly (I) and viral RNA.

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