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. 1999 Dec;43(12):2930-8.
doi: 10.1128/AAC.43.12.2930.

Multilocus genotypes and DNA fingerprints Do not predict variation in azole resistance among clinical isolates of Candida albicans

L E Cowen  1 , C Sirjusingh, R C Summerbell, S Walmsley, S Richardson, L M Kohn, J B Anderson
Affiliations

Multilocus genotypes and DNA fingerprints Do not predict variation in azole resistance among clinical isolates of Candida albicans

L E Cowen et al. Antimicrob Agents Chemother. 1999 Dec.

Abstract

If variation in azole resistance is due to inherent differences in strains of Candida albicans, as a predominantly clonal organism, then correlation between multilocus genotypes and drug resistance would be expected. A sample of 81 clinical isolates from patients infected with human immunodeficiency virus in Toronto, Canada, plus 3 reference isolates were genotyped at 16 loci, distributed on all linkage groups, by means of oligonucleotide hybridizations specific for each of the alleles at each locus. These multilocus genotypes were significantly correlated with DNA fingerprints obtained with the species-specific probe 27A, indicating widespread linkage disequilibrium in the genome. There were 64 multilocus diploid genotypes and 77 DNA fingerprint types delineated in this sample. Neither the multilocus genotyping nor DNA fingerprinting alone identified all of the 81 types identified by the combination of these two methods. Multilocus genotypes were not predictive of fluconazole resistance, suggesting that resistance is gained or lost too quickly to be predicted by linkage with neutral markers.

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Figures

FIG. 1
FIG. 1
Ordination plot depicting variation among multilocus genotypes. The x-axis explains 26.7% of the variation, and the y-axis explains 23.7% of the variation. The numerical symbol plotted for each isolate is the MIC of fluconazole, coded with a character from 0 to 9 on an exponential scale. The proximity of isolates in this plot represents similarity among genotypes but does not imply relatedness (i.e., patterns of descent).
FIG. 2
FIG. 2
Ordination plot representing variation among DNA fingerprints. The x-axis explains 12.4% of the variation, and the y-axis explains 9.5% of the variation. The numerical symbol plotted for each isolate is the MIC of fluconazole, coded with a character from 0 to 9 on an exponential scale. The proximity of isolates in this plot represents similarity among the hybridizing fragments of DNA fingerprints but does not imply relatedness (i.e., patterns of descent).
See this image and copyright information in PMC

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