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. 1975 Jul;72(7):2555-8.
doi: 10.1073/pnas.72.7.2555.

Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease

K Horiuchi, N D Zinder

Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease

K Horiuchi et al. Proc Natl Acad Sci U S A. 1975 Jul.

Abstract

Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R-HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.

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References

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    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed
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