Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Aug;72(8):3065-7.
doi: 10.1073/pnas.72.8.3065.

Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes

G Marbaix, G Huez, A Burny, Y Cleuter, E Hubert, M Leclercq, H Chantrenne, H Soreq, U Nudel, U Z Littauer

Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes

G Marbaix et al. Proc Natl Acad Sci U S A. 1975 Aug.

Abstract

Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nat New Biol. 1973 Nov 28;246(152):99-102 - PubMed
    1. J Mol Biol. 1973 Nov 5;80(3):539-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3143-6 - PubMed
    1. J Mol Biol. 1974 Sep 5;88(1):233-45 - PubMed
    1. Cell. 1974 Jul;2(3):189-96 - PubMed

Publication types

LinkOut - more resources