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. 1975 Aug;72(8):3065-7.
doi: 10.1073/pnas.72.8.3065.

Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes

G Marbaix, G Huez, A Burny, Y Cleuter, E Hubert, M Leclercq, H Chantrenne, H Soreq, U Nudel, U Z Littauer

Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes

G Marbaix et al. Proc Natl Acad Sci U S A. 1975 Aug.

Abstract

Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.

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References

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