The 1999 SWISS-2DPAGE database update

Abstract

SWISS-2DPAGE (http://www.expasy.ch/ch2d/ ) is an annotated two-dimensional polyacrylamide gel electro-phoresis (2-DE) database established in 1993. The current release contains 24 reference maps from human and mouse biological samples, as well as from Saccharomyces cerevisiae, Escherichia coli and Dictyostelium discoideum origin. These reference maps have now 2824 identified spots, corresponding to 614 separate protein entries in the database, in addition to virtual entries for each SWISS-PROT sequence or any user-entered amino acids sequence. Last year improvements in the SWISS-2DPAGE database are as follows: three new maps have been created and several others have been updated; cross-references to newly built federated 2-DE databases have been added; new functions to access the data have been provided through the ExPASy proteomics server.

Figure 1

Example of a SWISS-2DPAGE entry.

Figure 2

The protein from Figure 1 shown on the SWISS-2DPAGE E.coli reference map for pI range from 5 to 6 (ECOLI5–6). The protein spot itself is highlighted in red, and the red rectangle around shows the theoretical region where one would expect to see the protein, computed from the SWISS-PROT protein sequence.

Figure 3

Species distribution of the SWISS-2DPAGE entries. Abbreviations as follows: E.COLI, E.coli; YEAST, S.cerevisiae; DICTYSLUG, D.discoideum.