Perinatal exposure to the estrogenic pollutant bisphenol A affects behavior in male and female rats

Abstract

Bisphenol A (BPA) is an environmental estrogen with potentially aversive effects on public health. In rats, we studied the effects of perinatal exposure to BPA on nonsocial behaviors partly influenced by gonadal hormones. BPA was administered orally to one group of mother rats at a concentration within the range of human exposure from 10 days before mating until the weaning of the pups. In a second group, BPA was given at a higher dosage during a critical period for brain organization, i.e., from day 14 of gestation until day 6 after birth. The offspring of the treated mothers were tested in the holeboard and the elevated plus-maze at 85 days of age. Various aspects of nonsocial behavior were affected by BPA, differently in males and females, confirming that exposure to a weak environmental estrogen in the period of sexual differentiation of the brain can influence adult behavior. However, contrary to our expectation, a clear masculinization of females was not observed. In general, the factor analysis indicated that in treated males both the motivation to explore and anxiety are reduced, while in females, motor activity and motivation to explore are depressed. Because there were no substantial differences between the two modalities of BPA administration, we suggest that the prolonged treatment with the low dosage compensates for the higher dosage given during a shorter steroid-sensitive period. This may be a cause of concern for public health, given the greater incidence of prolonged exposure of humans to low concentrations released into the environment.