doi: 10.1093/genetics/90.4.673.
Regulation of newly evolved enzymes. IV. Directed evolution of the Ebg repressor
- PMID: 105963
- PMCID: PMC1213912
- DOI: 10.1093/genetics/90.4.673
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Regulation of newly evolved enzymes. IV. Directed evolution of the Ebg repressor
Genetics.
1978 Dec.
Abstract
In Escherichia coli, the wild-type repressor of ebg (evolved beta-galactosidase) enzyme synthesis, specified by the ebgR+ gene, responds very weakly to lactulose (fructose-beta-D-galactopyranoside). Selection for a functional repressor that responds strongly to lactulose as an inducer reveals the existence of ebgR+L mutants, which occur spontaneously at a frequency of about 2 X 10(-10) . EBGR+L mutants are pleiotropic in that they specify ebg repressor with a greatly increased response to lactulose, lactose, galactose-arabinoside and methyl-galactoside as inducers. Selection of ebgR+L mutants is discussed within the framework of directed evolution of a regulatory function.
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