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. 1999 Sep;12(2):203-6.
doi: 10.1385/MB:12:2:203.

An antibody-lectin sandwich assay for quantifying protein glycoforms

F T Lundy  1 , G B Wisdom
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An antibody-lectin sandwich assay for quantifying protein glycoforms

F T Lundy et al. Mol Biotechnol. 1999 Sep.

Abstract

We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein's glycoforms by simply substituting the antibody and lectin with specific alternatives.

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