doi: 10.1073/pnas.72.10.4028.
Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells
- PMID: 1060086
- PMCID: PMC433131
- DOI: 10.1073/pnas.72.10.4028
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Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells
Proc Natl Acad Sci U S A.
1975 Oct.
Abstract
Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.
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