Functional level of rat liver tryptophan 2,3-dixoygenase messenger RNA during superinduction of enzyme with actinomycin D
- PMID: 1060107
- PMCID: PMC388705
- DOI: 10.1073/pnas.72.11.4285
Functional level of rat liver tryptophan 2,3-dixoygenase messenger RNA during superinduction of enzyme with actinomycin D
Abstract
Tryptophan 2,3-dioxygenase [EC 1.13.11.11; L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing)] activity is induced by glucocorticoid hormones and superinduced by actinomycin D. Previous experiments had shown that hormonal induction of the enzyme activity is accompanied by parallel increases in tryptophan 2,3-dioxygenase mRNA level. In this study, we measured the tryptophan 2,3-dioxygenase mRNA levels during superinduction as well as hormonal induction, to determine whether superinduction of the enzyme activity is also mediated through changes in mRNA concentration. Tryptophan 2,3-dioxygenase mRNA was measured in a Krebs ascites cell-free protein synthesizing system supplemented with rabbit reticulocyte initiation factors. We found that during superinduction of the enzyme activity by actinomycin D, the mRNA level is identical to that of the actinomycin D-free controls. Our results do not, therefore, support the hypothesis that hormonal induction and/or superinduction of tryptophan 2,3-dioxygenase mRNA are regulated by a rapidly turning over repressor.
Similar articles
-
Developmental control of messenger RNA for hepatic tryptophan 2,3-dioxygenase.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5392-6. doi: 10.1073/pnas.74.12.5392. Proc Natl Acad Sci U S A. 1977. PMID: 271960 Free PMC article.
-
Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.Proc Natl Acad Sci U S A. 1975 Mar;72(3):1017-20. doi: 10.1073/pnas.72.3.1017. Proc Natl Acad Sci U S A. 1975. PMID: 1055360 Free PMC article.
-
Inhibition by actinomycin D of the induction of tryptophan pyrrolase by hydrocortisone.Biochim Biophys Acta. 1967 Mar 29;138(1):169-74. doi: 10.1016/0005-2787(67)90597-7. Biochim Biophys Acta. 1967. PMID: 6048280 No abstract available.
-
Studies on the hepatic glucocorticoid receptor and on the hormonal modulation of specific mRNA levels during enzyme induction.Recent Prog Horm Res. 1975;31:213-42. doi: 10.1016/b978-0-12-571131-9.50010-4. Recent Prog Horm Res. 1975. PMID: 172993 Review. No abstract available.
-
Relationship between interferon-gamma, indoleamine 2,3-dioxygenase, and tryptophan catabolism.FASEB J. 1991 Aug;5(11):2516-22. FASEB J. 1991. PMID: 1907934 Review.
Cited by
-
Superinduction of alpha 2u globulin by actinomycin D: evidence for drug-mediated increase in alpha 2u mRNA.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1833-7. doi: 10.1073/pnas.76.4.1833. Proc Natl Acad Sci U S A. 1979. PMID: 88049 Free PMC article.
-
Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production.Proc Natl Acad Sci U S A. 1977 Aug;74(8):3409-13. doi: 10.1073/pnas.74.8.3409. Proc Natl Acad Sci U S A. 1977. PMID: 269400 Free PMC article.
-
Identification, Bioactivity, and Productivity of Actinomycins from the Marine-Derived Streptomyces heliomycini.Front Microbiol. 2017 Jun 28;8:1147. doi: 10.3389/fmicb.2017.01147. eCollection 2017. Front Microbiol. 2017. PMID: 28702007 Free PMC article.
-
Developmental control of messenger RNA for hepatic tryptophan 2,3-dioxygenase.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5392-6. doi: 10.1073/pnas.74.12.5392. Proc Natl Acad Sci U S A. 1977. PMID: 271960 Free PMC article.
-
Thionein gene expression in Cd++-variants of the CHO cell: correlation of thionein synthesis rates with translatable mRNA levels during induction, deinduction, and superinduction.Nucleic Acids Res. 1979 Sep 11;7(1):271-88. doi: 10.1093/nar/7.1.271. Nucleic Acids Res. 1979. PMID: 493142 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
