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. 1975 Nov;72(11):4394-8.
doi: 10.1073/pnas.72.11.4394.

Initiation of in vitro mRNA synthesis from the wild-type lac promoter

J Majors

Initiation of in vitro mRNA synthesis from the wild-type lac promoter

J Majors. Proc Natl Acad Sci U S A. 1975 Nov.

Abstract

An in vitro transcription system, dependent on catabolite gene activator protein (CAP), utilizing a 200 base-pair restriction fragment, has been used to show that the initiation site of the wild-type Escherichia coli lac mRNA, and that of two mutants, 8d and ps, are identical to that previously reported for the CAP-independent promoter mutant UV5. Order of addition experiments are used to show that the binding of lac repressor to the operator is competitive with that of the RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to the promoter, thus demonstrating functional overlap of the operator and promoter sites.

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