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. 1975 Nov;72(11):4404-8.
doi: 10.1073/pnas.72.11.4404.

Structure of transcriptionally active chromatin

J M Gottesfeld, R F Murphy, J Bonner

Structure of transcriptionally active chromatin

J M Gottesfeld et al. Proc Natl Acad Sci U S A. 1975 Nov.

Abstract

Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by tne endonuclease DNase II. The nuclease-resistant structures of inactive chromatin are DNA-histone complexes (v-bodies) which sediment at 11-13 S. Template-active chromatin yields two peaks of nuclease-resistant nucleoprotein. These complexes sediment at 14 and 19 S, and contain DNA, RNA, histone, and nonhistone chromosomal proteins. Polyacrylamide gel electrophoresis reveals a complex pattern of chromatin proteins, suggesting that the complexes are heterogeneous in composition.

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References

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