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. 1975 Nov;72(11):4597-601.
doi: 10.1073/pnas.72.11.4597.

Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles

J Summers, A O'Connell, I Millman

Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles

J Summers et al. Proc Natl Acad Sci U S A. 1975 Nov.

Abstract

DNA extracted from Dane particles has been characterized by gel electrophoresis and restriction enzyme cleavage with endonuclease R-HaeIII (from Hemophilus aegyptius). Dane particle DNA is proposed to be a double-stranded circular DNA approximately 3600 nucleotides in length containing a single-stranded gap of 600-2100 nucleotides. The endogenous DNA polymerase (DNA nucleotidyl-transferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 2.7.7.7) reaction appears to repair this single-stranded gap.

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