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. 1999 Oct;15(2):181-4.
doi: 10.1023/a:1008358030477.

Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments

M Salzmann  1 , G Wider, K Pervushin, K Wüthrich
Affiliations

Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments

M Salzmann et al. J Biomol NMR. 1999 Oct.

Abstract

In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.

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