Utilization of highly deoxynivalenol-contaminated wheat via extrusion processing

Abstract

Deoxynivalenol (DON) is a toxic natural metabolite produced by Fusarium graminearum. In this study we investigated the effects of sodium bisulfite and extrusion cooking under high temperature and pressure on DON levels in wheat grain and mill fractions. Samples of highly naturally DON-contaminated soft winter wheat were soaked for 1 h in water or aqueous sodium bisulfite (SB) solutions (0.5, 1.5, 2.5, or 5% SO2 equivalent) and extruded. The soaking treatment with SB solution (5% SO2 equivalent) lowered DON from 7.3 microg/g to 0.8 microg/g without extrusion and to 0.3 microg/g with an extrusion process. When the contaminated kernels were tempered with water or SB solutions (5 or 10% SO2 equivalent) and milled, the flour samples, as expected, showed lower levels of DON (from 7.3 to 3.1 microg/g). Extrusion of milled flour and whole meal samples, both obtained from SB-tempered wheat, did not change DON levels significantly under the studied extrusion conditions as compared to the nonextruded milled flour and whole meal samples. However, the extrusion process can be utilized to remove moisture and/or odor of chemicals used in the soaking solutions and to produce potentially useful extruded products.