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Review
. 2000 Jan;182(1):9-13.
doi: 10.1128/JB.182.1.9-13.2000.

Role of PriA in replication fork reactivation in Escherichia coli

S J Sandler  1 , K J Marians
Affiliations
Review

Role of PriA in replication fork reactivation in Escherichia coli

S J Sandler et al. J Bacteriol. 2000 Jan.
No abstract available

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Figures

FIG. 1
FIG. 1
PriA-directed replisome assembly at a D loop. (Top) Relative positions of PriA and DnaB on D-loop DNA during assembly of the primosome, as deduced from DNA footprinting studies. (Bottom) Direction of subsequent replication fork progression. The invading strand is used as the primer for leading-strand synthesis, whereas Okazaki fragment synthesis is primed as a result of the periodic interaction of DnaG with DnaB as the helicase moves 5′→3′ along the lagging-strand template.
FIG. 2
FIG. 2
Multiple pathways of replication fork assembly at recombination intermediates. One possible set of pathways, based on the genetic data discussed in the text, involves the replication restart primosomal proteins and Rep during replication fork assembly at recombination intermediates. Each column denotes one possible pathway. The steps separated by vertical arrows in each column represent the order of action of particular proteins during assembly of the restart primosome. This order of assembly is based on biochemical data discussed in the text. The bifurcation in the PriA-dependent pathway indicates that PriA can interact with either PriB or PriC (and Rep?). Question marks indicate that the action of this protein in the pathway is unclear. Regardless of which proteins are present on the recombination intermediate as a final protein-DNA complex, the object of each pathway is to place DnaB in position on single-stranded DNA so that a new replication fork can form.
See this image and copyright information in PMC

References

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