Recovery of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Israel

Abstract

Candida dubliniensis is a recently discovered yeast species principally associated with carriage and disease in the oral cavities of human immunodeficiency virus (HIV)-infected individuals. To date the majority of isolates of this species have been identified in Europe and North America. In this study, five Candida isolates recovered from separate HIV-negative hospitalized patients in Jerusalem, Israel, were presumptively identified as C. dubliniensis on the basis of their dark green coloration when grown on CHROMagar Candida medium. Their identification was confirmed by a variety of techniques, including carbohydrate assimilation profiles, absence of growth at 45 degrees C, positive reaction with C. dubliniensis-specific antibodies as determined by indirect immunofluorescence analysis, and positive amplification with C. dubliniensis-specific PCR primers. All five strains were shown to be susceptible to a range of antifungal agents, including fluconazole. One of the five isolates was recovered from urine specimens, while the remaining four were recovered from upper respiratory tract and oral samples. While none of the patients was HIV positive, all were receiving broad-spectrum antibacterials at the time isolates of C. dubliniensis were obtained from clinical specimens. This study describes the first isolates of C. dubliniensis from the Middle East and confirms that this yeast can be associated with carriage and infection in the absence of HIV infection.

FIG. 1

Agarose gel showing ethidium bromide-stained amplimers from PCRs with the C. dubliniensis-specific primers DUBF and DUBR (288-bp product) and the fungal universal primers RNAF and RNAR (610-bp product). The amplimers shown in the lanes were obtained from template DNA from yeast strains and isolates as follows: lane 2, C. dubliniensis type strain CD36; lane 3, C. albicans reference strain 132A, lanes 4 to 8, Israeli C. dubliniensis isolates P-6265, P-6785, P-7073, P-7266, and P-7276, respectively. Lane 1, molecular weight size markers (100-bp ladder); lane 9, negative control lacking template DNA.