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. 2000 Jan;38(1):292-300.
doi: 10.1128/JCM.38.1.292-300.2000.

Molecular methods for the epidemiological typing of Salmonella enterica serotype Typhi from Hong Kong and Vietnam

J M Ling  1 , N W Lo, Y M Ho, K M Kam, N T Hoa, L T Phi, A F Cheng
Affiliations

Molecular methods for the epidemiological typing of Salmonella enterica serotype Typhi from Hong Kong and Vietnam

J M Ling et al. J Clin Microbiol. 2000 Jan.

Abstract

A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with PstI, and total DNA fingerprinting with NarI. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi.

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Figures

FIG. 1
FIG. 1
Plasmid profiles of S. enterica serotype Typhi from Vietnam. Lanes 1 to 5, 16, 17, and 20, plasmids from chloramphenicol-resistant isolates; lanes 6, 7, 12 to 15, 18, and 19, plasmids from chloramphenicol- and tetracycline-resistant isolates. Size markers are given in lanes 8 (49 kb), 9 (supercoiled DNA ladder [GIBCO BRL, Gaithersburg, Md.]), 10 (91 and 4.4 kb), and 11 (221 and 9 kb). Lanes 21 to 26 and 33 to 42, plasmids from sensitive isolates. Size markers are given in lanes 27 (91 kb), 28 (58 kb), 29 (8.8 kb), 30 (119 kb), 31 (4.4 kb), and 32 (5.4 kb).
FIG. 2
FIG. 2
EcoRI digests of 221- and 98-kb plasmids of S. enterica serotype Typhi from Vietnam. Lanes 1 to 5, transferable 221-kb plasmid from chloramphenicol- and tetracycline-resistant isolates; lanes 7 to 12, nontransferable 98-kb plasmids from chloramphenicol-resistant isolates; lanes 14 to 20, transferable 98-kb plasmids from chloramphenicol-resistant isolates; lanes 22 to 28, 98-kb plasmids from sensitive isolates; lanes 6, 13, and 21, HindIII-digested bacteriophage λ DNA as a size marker.
FIG. 3
FIG. 3
HindIII digests of 221- and 98-kb plasmids of S. enterica serotype Typhi from Vietnam. The contents of the lanes are described in the legend to Fig. 2.
FIG. 4
FIG. 4
PstI ribotypes of S. enterica serotype Typhi (lanes 1 to 14 and 16 to 26) and HindIII-digested bacteriophage λ DNA as a negative control (lane 15).
FIG. 5
FIG. 5
Dendrogram showing relatedness of PstI ribotypes.
FIG. 6
FIG. 6
Total DNA fingerprints of S. enterica serotype Typhi obtained after NarI digestion (lanes 1 to 9 and 11 to 21) and high-molecular-size markers of 48.5, 38.4, 33.5, 29.9, 24.8, 22.6, 19.4, 17.1, 15, 12.2, and 10 kb from GIBCO BRL (Gaithersburg, Md.) (lane 10).
FIG. 7
FIG. 7
Dendrogram showing relatedness of NarI total DNA fingerprints.
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