Pulsed-field gel electrophoresis-based molecular comparison of vibrio cholerae O1 isolates from domestic and imported cases of cholera in Japan

Abstract

Sixty-seven Vibrio cholerae O1 El Tor isolates (36 domestic and 31 imported) were classified into 19 subtypes by NotI- and SfiI-digested pulsed-field gel electrophoresis. Twenty-five of 36 domestic and 4 imported isolates were assigned to a NotI-A1-SfiI-A1 subtype, suggesting that this pulse type is widely distributed in Asia and Japan.

FIG. 1

Representative PFGE patterns of V. cholerae O1 strains cleaved with restriction enzyme NotI. Lanes A to K show the representative PFGE patterns of the types and subtypes (A1, B1, C1, D, E2, F1, J, and K, respectively) shown in Table 1 (D, F1, and J were derived from isolates of domestic outbreaks during the past 2 decades). λ is a 48.5-kb ladder size marker. The values on the right are sizes in kilobases.