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Clinical Trial
. 2000 Jan;38(1):444-7.
doi: 10.1128/JCM.38.1.444-447.2000.

Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence In situ hybridization probes

F A Drobniewski  1 , P G More, G S Harris
Affiliations
Clinical Trial

Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence In situ hybridization probes

F A Drobniewski et al. J Clin Microbiol. 2000 Jan.

Abstract

A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81. 5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium flavescens.

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References

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