Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Mar;73(3):717-21.
doi: 10.1073/pnas.73.3.717.

Determination of nucleotide sequences beyond the sites of transcriptional termination

M Rosenberg, B de Chrombrugghe, R Musso

Determination of nucleotide sequences beyond the sites of transcriptional termination

M Rosenberg et al. Proc Natl Acad Sci U S A. 1976 Mar.

Abstract

A procedure is described by which a discrete high-molecular-weight RNA transcription product can be used as a primer by DNA polymerase (DNA nucleotidyltransferase; EC 2.7.7.7; deoxynucleoside triphosphate: DNA deoxynucleotidyltransferase) for determining nucleic acid sequence in the template DNA beyond the 3'-terminus of the transcript. This procedure is applied to two lambda phage transcripts, the 4S "oop" RNA [Short l-strand RNA transcript from the region of origin of replication (ori) and the 6S RNA. Sequences of 35 and 19 nucleotides, respectively, following the sites at which these two transcripts terminate, are determined. Little structural homology is apparent in the template DNA beyond the 3'-ends of these two transcripts. The lack of homology suggests that this region might not be important to the termination process.

PubMed Disclaimer

References

    1. J Biol Chem. 1973 Jul 25;248(14):5024-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Feb;73(2):293-7 - PubMed
    1. J Mol Biol. 1974 Aug 15;87(3):377-407 - PubMed
    1. Arch Biochem Biophys. 1972 Sep;152(1):152-65 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3300-4 - PubMed

LinkOut - more resources