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. 1999;7(8):641-7.
doi: 10.1023/a:1009288119851.

A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes

S C Andras  1 , T P Hartman, J A Marshall, R Marchant, J B Power, E C Cocking, M R Davey
Affiliations

A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes

S C Andras et al. Chromosome Res. 1999.

Abstract

A preparation technique has been developed for plants with small chromosomes, which produces large numbers of good-quality mitotic preparations. The technique employs a hydrochloric acid treatment to hydrolyse the cytoplasm, facilitating the subsequent removal of cytoplasmic debris. The evaporative force of a methanol-based fixative is exploited to disperse the cytoplasm and to deposit the chromosomes in a single optical plane. This technique permits detailed observations of chromosome morphology and karyotyping. The mitotic preparations are also suitable for the complex analysis associated with in-situ hybridization, as in studies of genome interaction in plant hybrids.

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References

    1. Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 - PubMed
    1. Cytometry. 1986 May;7(3):235-42 - PubMed
    1. Biotech Histochem. 1998 May;73(3):150-6 - PubMed
    1. Theor Appl Genet. 1991 May;81(5):597-605 - PubMed
    1. Biotech Histochem. 1997 May;72(3):141-3 - PubMed

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