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. 1999 Dec;262(4-5):668-76.
doi: 10.1007/s004380051129.

A new transcriptional activator for amylase genes in Aspergillus

K L Petersen  1 , J Lehmbeck, T Christensen
Affiliations

A new transcriptional activator for amylase genes in Aspergillus

K L Petersen et al. Mol Gen Genet. 1999 Dec.

Abstract

We have cloned a regulatory gene for amylase synthesis in Aspergillus oryzae. This gene, amyR, encodes a 604-amino acid transcriptional activator with a Cys6 zinc cluster, that shows extensive homology to the DNA binding domain of GAL4 from Saccharomyces cerevisiae. The DNA binding domain of amyR binds to two types of sequences found in a number of promoters from Aspergillus genes coding for starch-degrading enzymes. One type of binding site is characterized by two CGG triplets separated by eight nucleotides. The other type has only one CGG triplet, which is followed by the sequence AAATTTAA.

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