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. 2000 Feb;182(3):825-8.
doi: 10.1128/JB.182.3.825-828.2000.

Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11

R Hirota  1 , A Yamagata, J Kato, A Kuroda, T Ikeda, N Takiguchi, H Ohtake
Affiliations

Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11

R Hirota et al. J Bacteriol. 2000 Feb.

Abstract

Pulsed-field gel electrophoresis of PmeI digests of the Nitrosomonas sp. strain ENI-11 chromosome produced four bands ranging from 1,200 to 480 kb in size. Southern hybridizations suggested that a 487-kb PmeI fragment contained two copies of the amoCAB genes, coding for ammonia monooxygenase (designated amoCAB(1) and amoCAB(2)), and three copies of the hao gene, coding for hydroxylamine oxidoreductase (hao(1), hao(2), and hao(3)). In this DNA fragment, amoCAB(1) and amoCAB(2) were about 390 kb apart, while hao(1), hao(2), and hao(3) were separated by at least about 100 kb from each other. Interestingly, hao(1) and hao(2) were located relatively close to amoCAB(1) and amoCAB(2), respectively. DNA sequence analysis revealed that hao(1) and hao(2) shared 160 identical nucleotides immediately upstream of each translation initiation codon. However, hao(3) showed only 30% nucleotide identity in the 160-bp corresponding region.

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Figures

FIG. 1
FIG. 1
PFGE of PmeI, XbaI, and AscI digests of Nitrosomonas sp. strain ENI-11 genomic DNA (A) and Southern hybridization analysis of the digested genomic DNA with the amoB (B) and hao (C) probes. Lanes: a, PmeI digestion; b, XbaI digestion; c, AscI digestion. The size markers (lane M in panel A) are the concatemers of lambda DNA (FMC).
FIG. 2
FIG. 2
AscI and XbaI restriction map of the PmD fragment from the ENI-11 genome. The locations and directions of the multiple copies of amoCAB and hao are shown by white and black arrowheads, respectively. Plasmids pHP1 and pAX1 were used for constructing additional PmeI sites in the PmD fragment. The locations of the pHP1 and pAX1 insertions are indicated by arrows.
See this image and copyright information in PMC

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