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. 1999 Dec;37(4):243-8.
doi: 10.3347/kjp.1999.37.4.243.

Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis

S T Hong  1 , M Lee, N J Sung, S R Cho, J Y Chai, S H Lee
Affiliations

Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis

S T Hong et al. Korean J Parasitol. 1999 Dec.

Abstract

The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and IgG2 antibodies were produced but not specific, IgG3 antibody was least produced, and IgG4 antibody was prominent and specific. The serum IgG antibody reaction to any of 43-50, 34-37, 26-28, and 8 kDa bands was found in 65.5% of 168 egg positive cases while IgG4 antibody reaction was found in 22.0% of them. The positive rates of IgG and IgG4 antibodies were directly correlated with the intensity of infection. All of the sera from heavily infected cases over EPG 5,000 showed positive reaction for specific IgG and IgG4 antibodies. The specific serum IgG4 antibody disappeared within 6 months after treatment. The bands of 35 kDa and 67 kDa cross-reacted with IgG antibodies but not with IgG4 antibodies in sera of other trematode infections. The present findings suggest that serum IgG4 antibody reaction to 8 kDa band is specific but not sensitive. Any method to increase its sensitivity is required for improved serodiagnosis.

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Figures

Fig. 1
Fig. 1
Immunoblot analysis of IgG antibodies and IgG subclass antibodies of Clonorchis sinensis infected sera. Lanes 1-5, IgG antibodies; lanes 6-10, IgG1; lanes 11-15, IgG2; lanes 16-20, IgG3; lanes 21-25, IgG4 subclass antibodies.
Fig. 2
Fig. 2
Cross-reaction with sera of other parasite infections. A. IgG antibody reaction. B. IgG4 antibody reaction. lanes 1-3, cysticercus of Taenia solium; lanes 4-17, Paragonimus westermani; lanes 18-24, Gymnophalloides seoi; lane 25, sparganum; lane 26, Fasciola hepatica. The 35 and 67 kDa bands are reacting to serum IgG antibodies of other parasite infections but no bands reacted to IgG4 antibodies.
Fig. 3
Fig. 3
Immunoblot analysis of serum IgG and IgG4 antibodies of Clonorchis sinensis infected cases, paired samples after treatment. Lanes 1-10, IgG antibodies in paired sera pre- (odd numbers) and post-treatment (even numbers) of 5 cases; lanes 11-20, IgG4 antibodies in paired sera pre- (odd numbers) and post-treatment (even numbers) of 5 cases.
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