doi: 10.1073/pnas.73.4.1314.
Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues
- PMID: 1063412
- PMCID: PMC430258
- DOI: 10.1073/pnas.73.4.1314
Item in Clipboard
Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues
Proc Natl Acad Sci U S A.
1976 Apr.
Abstract
A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.
Similar articles
-
Oxytocin and vasopressin are present in human and rat pancreas.Am J Med Sci. 1988 Nov;296(5):303-7. doi: 10.1097/00000441-198811000-00003. Am J Med Sci. 1988. PMID: 3195625
-
Vasopressin and oxytocin in the developing rat brain as shown by isoelectric focusing of radioimmunoassayable peptides.Brain Res. 1986 Apr;391(1):85-90. doi: 10.1016/0165-3806(86)90010-6. Brain Res. 1986. PMID: 3955382
-
Neurohypophysial peptides in the bovine hypothalamus: the relationship of neurophysin I to oxytocin, and neurophysin II to vasopressin in supraoptic and paraventricular regions.Endocrinology. 1974 Oct;95(4):931-6. doi: 10.1210/endo-95-4-931. Endocrinology. 1974. PMID: 4414801 No abstract available.
-
The radioimmunoassay of oxytocin and vasopressin.J Endocrinol. 1973 Jul;58(1):143-60. doi: 10.1677/joe.0.0580143. J Endocrinol. 1973. PMID: 4578963 Review. No abstract available.
-
The neurohypophysis: recent developments.J Lab Clin Med. 1987 Mar;109(3):336-45. J Lab Clin Med. 1987. PMID: 3029255 Review.
Cited by
-
Neurohypophyseal vasopressor principle: vasopressor hormone as well as antidiuretic hormone?Klin Wochenschr. 1978;56 Suppl 1:71-9. doi: 10.1007/BF01477456. Klin Wochenschr. 1978. PMID: 732254
-
The Research Progress of Bioactive Peptides Derived from Traditional Natural Products in China.Molecules. 2023 Sep 3;28(17):6421. doi: 10.3390/molecules28176421. Molecules. 2023. PMID: 37687249 Free PMC article. Review.
-
A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues.Biochem J. 1978 Dec 1;175(3):1139-41. doi: 10.1042/bj1751139. Biochem J. 1978. PMID: 233923 Free PMC article.
-
Chemical determination of polypeptide hormones.Proc Natl Acad Sci U S A. 1978 Sep;75(9):4115-9. doi: 10.1073/pnas.75.9.4115. Proc Natl Acad Sci U S A. 1978. PMID: 279902 Free PMC article.
-
Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052-5. doi: 10.1073/pnas.74.7.3052. Proc Natl Acad Sci U S A. 1977. PMID: 197530 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
