Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jan;6(1):27-39.
doi: 10.1093/nar/6.1.27.

The primary transcript of the ribosomal repeating unit in yeast

J Klootwijk, P de Jonge, R J Planta

The primary transcript of the ribosomal repeating unit in yeast

J Klootwijk et al. Nucleic Acids Res. 1979 Jan.

Abstract

Endgroup analysis of 37S ribosomal precursor RNA from Saccharomyces carlsbergensis has revealed that the major 5' endgroup is ppA-Up, with a molar yield of 0.8. This shows that most, if not all, 37S RNA molecules have preserved a transcriptional initiation sequence. Analysis of the 3' terminus of 37S RNA has shown the presence of a uridine rich oligonucleotide, tentatively identified as U6-8-A-NOH. This long stretch of uridines at the 3' end of 37S RNA may represent a transcriptional termination site. The two sets of data on the terminal sequences suggest that 37S ribosomal precursor RNA, if not already spliced, is a primary transcription product. Since the 3' terminus of 26S rRNA, U-U-U-G-UOH., appears to be clearly different from the 3' end of 37S RNA, we conclude that 37S ribosomal precursor RNA contains additional nucleotides 3'-distal to the 26S rRNA sequence.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1974 May;1(5):653-71 - PubMed
    1. Exp Cell Res. 1975 Jan;90(1):95-104 - PubMed
    1. Biochim Biophys Acta. 1975 Jun 16;395(2):179-90 - PubMed
    1. Biochim Biophys Acta. 1975 Dec 4;414(2):115-25 - PubMed
    1. Mol Biol Rep. 1975 Dec;2(4):321-5 - PubMed