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. 1979 Feb;76(2):726-30.
doi: 10.1073/pnas.76.2.726.

A protein that preferentially binds Drosophila satellite DNA

T Hsieh, D L Brutlag

A protein that preferentially binds Drosophila satellite DNA

T Hsieh et al. Proc Natl Acad Sci U S A. 1979 Feb.

Abstract

Using a nitrocellulose filter binding assay, we have detected and partially purified a protein from embryos of Drosophila melanogaster that preferentially binds to a highly repeated satellite DNA of the same species. Formation of the satellite DNA-protein complex requires physiological conditions of salt and temperature, but once formed, the complex is stable in high salt (1 M NaCl) or at low temperature. Optimal formation of the specific complex also requires the satellite DNA to be in a supertwisted conformation. The protein interacts with a limited region within the 359-base-pair repeated sequence of the satellite DNA.

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References

    1. Science. 1975 Jan 10;187(4171):27-35 - PubMed
    1. Biochemistry. 1975 Feb 11;14(3):527-35 - PubMed
    1. J Mol Biol. 1975 Aug 25;96(4):693-702 - PubMed
    1. J Biol Chem. 1976 Apr 10;251(7):2124-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Nov;52:1248-55 - PubMed

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