Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Feb;44(2):453-5.
doi: 10.1128/AAC.44.2.453-455.2000.

Characterization of TEM-56, a novel beta-lactamase produced by a Klebsiella pneumoniae clinical isolate

C Neuwirth  1 , R Labia, E Siebor, A Pechinot, S Madec, E B Chaibi, A Kazmierczak
Affiliations

Characterization of TEM-56, a novel beta-lactamase produced by a Klebsiella pneumoniae clinical isolate

C Neuwirth et al. Antimicrob Agents Chemother. 2000 Feb.

Abstract

TEM-56 produced by a Klebsiella pneumoniae clinical isolate is a novel beta-lactamase of isoelectric point 6.4 that confers a moderate resistance level to expanded-spectrum cephalosporins. The amino acid sequence deduced from the corresponding bla gene showed two amino acid replacements with respect to the TEM-2 sequence: Glu-104 to Lys and His-153 to Arg. This enzyme showed catalytic properties close to those of TEM-18. Thus, TEM-56 appears as a new TEM mutant, an intermediary between TEM-18 and the extended-spectrum beta-lactamase TEM-21.

PubMed Disclaimer

References

    1. Ambler R P, Coulson A F W, Frère J M, Ghuysen J M, Joris B, Forsman M, Levesque R C, Tirabi G, Waley S G. A standard numbering scheme for the class A β-lactamases. Biochem J. 1991;276:269–272. - PMC - PubMed
    1. Arlet G, Brami G, Decré D, Filippo A, Gaillot O, Lagrange P H, Philippon A. Molecular characterisation by PCR-restriction fragment length polymorphism of TEM β-lactamases. FEMS Microbiol Lett. 1995;134:203–208. - PubMed
    1. Arlet G, Goussard S, Courvalin P, Philippon A. Sequences of the genes for the TEM-20, TEM-21, TEM-22, and TEM-29 extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1999;43:969–971. - PMC - PubMed
    1. Birnboim H C, Doly J. Rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7:1513–1523. - PMC - PubMed
    1. Cantu C, III, Huang W, Palzkill T. Selection and characterization of amino acid substitutions at residues 237-240 of TEM-1 β-lactamase with altered substrate specificity for aztreonam and ceftazidime. J Biol Chem. 1996;271:22538–22545. - PubMed

LinkOut - more resources