Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jun;73(6):2023-7.
doi: 10.1073/pnas.73.6.2023.

Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts

W L McKeehan, W G Hamilton, R G Ham

Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts

W L McKeehan et al. Proc Natl Acad Sci U S A. 1976 Jun.

Abstract

The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium.

PubMed Disclaimer

References

    1. Can Vet J. 1964 Oct;5(10):268-73 - PubMed
    1. J Exp Med. 1956 Feb 1;103(2):273-83 - PubMed
    1. Exp Cell Res. 1965 Mar;37:614-36 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 - PubMed
    1. Exp Cell Res. 1963 Feb;29:515-26 - PubMed

Publication types

LinkOut - more resources