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. 2000 Feb;66(2):783-7.
doi: 10.1128/AEM.66.2.783-787.2000.

Immunolocalization of dinitrogenase reductase produced by Klebsiella pneumoniae in association with Zea mays L

M K Chelius  1 , E W Triplett
Affiliations

Immunolocalization of dinitrogenase reductase produced by Klebsiella pneumoniae in association with Zea mays L

M K Chelius et al. Appl Environ Microbiol. 2000 Feb.

Abstract

The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat for K. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.

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Figures

FIG. 1
FIG. 1
Maize stem (a) and root (b to d) colonization by GFP-labeled K. pneumoniae strain 2028 (a and b) or 342 (c and d). Sections were visualized 8 (a) or 4 (b to d) days after inoculation. (a) Transverse section of a maize stem (red) colonized by K. pneumoniae (yellow-green). (b to d) Longitudinal section of a maize root (dark green) colonized by GFP-tagged cells (bright green). Arrows point to GFP-tagged cells. Bar, 20 (a and b) or 10 (c and d) μm.
FIG. 2
FIG. 2
Immunolocalization of NifH produced by K. pneumoniae 2028 in maize roots. All images are longitudinal sections. (a to c) Series of images demonstrating fluorescence generated by green GFP-labeled cells (a), red NifH-producing cells (b), and a combination of the preceding images resulting in yellow cells (c). Immunolocalization of NifH at 3 (d), 4 (e), and 8 (f) days after inoculation. Bar, 10 (a to c), 20 (d, e, and f), or 50 (d offset) μm.
See this image and copyright information in PMC

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