PCR-Based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells

Abstract

An assay that quantifies the amount of human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood mononuclear cells has been developed. PCR amplification of the HIV-1 DNA is performed in the presence of an internal quantitation standard, and colorimetric detection of the amplified product is performed with microwell plates. The copies of HIV-1 DNA are normalized to total genomic DNA input. The assay has an analytical sensitivity of 10 input copies per amplification reaction and a three-log detection range. In an analysis of sequential samples from patients on combination therapy, HIV-1 DNA was quantifiable for all individuals tested, including those with undetectable plasma HIV-1 RNA. In a separate study, a comparison of HIV-1 DNA levels was made with a group of long-term survivors and progressors. The mean HIV-1 DNA levels were lower in the long-term survivors than in the progressors (P, 0.04). The mean HIV-1 RNA levels were also lower, but the difference was not statistically significant (P, 0.164). A quantitative DNA assay will provide an additional tool to gain insight into the natural history of infection and the continued efficacy of potent antiretroviral therapies.

FIG. 1

Longitudinal evaluation of HIV-1 RNA and DNA levels in the circulation of two patients on HAART with zidovudine, didanosine, and nevirapine. The solid line represents HIV-1 DNA in copies per microgram, and the broken line represents HIV-1 RNA levels in copies per milliliter. The solid horizontal line represents the 50-copy/ml cutoff for HIV-1 RNA levels.