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Case Reports
. 2000 Feb;38(2):789-94.
doi: 10.1128/JCM.38.2.789-794.2000.

Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia

M M Kattar  1 , J F Chavez, A P Limaye, S L Rassoulian-Barrett, S L Yarfitz, L C Carlson, Y Houze, S Swanzy, B L Wood, B T Cookson
Affiliations
Case Reports

Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia

M M Kattar et al. J Clin Microbiol. 2000 Feb.

Abstract

We report on the first case of fatal septicemia caused by Bordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella avium with three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.

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Figures

FIG. 1
FIG. 1
Maximum likelihood consensus dendrogram based on 1,341 consecutive positions of 16S rRNA of bordetellae and related species. The lower bar indicates the genetic distance. The sequence accession numbers retrieved from GenBank for phylogenetic tree construction are as follows: B. bronchiseptica, U04948; B. parapertussis, U04949; B. pertussis, AF142326; B. holmesii, U04820; Achromobacter ruhlandii, AB010840; Achromobacter piechaudii, AB010841; Alcaligenes xylosoxidans subsp. xylosoxidans, M22509; Alcaligenes defragrans, AJ005450; Alcaligenes faecalis, M22508; Ralstonia eutropha, AF027407; Ralstonia solanacearum, X67036; CDC Group IV C-2, AF067657; and P. aeruginosa (used as the outgroup), Z76651.
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